| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1531 | Holdemania filiformis | TTGTCACGATGCTGCCGT | TGCAGCGAACACGACACA | 59.97 | 60.20 | 181 | 20 |
100.00%
|
50.00%
|
| Primer 1532 | Holdemania filiformis | AGCTCAGAATGCCGACGT | TGCCGTGCAGAATCAGCA | 59.03 | 59.97 | 299 | 20 |
100.00%
|
50.00%
|
| Primer 1533 | Holdemania filiformis | TGCCGAGCAAATGGTGGA | GCTGCATTGCTTCAGCGT | 59.57 | 59.44 | 172 | 20 |
100.00%
|
50.00%
|
| Primer 1534 | Holdemania filiformis | TTACCGCAGCGTTTCCGT | ACCGATCAGCGAACCGTT | 59.97 | 59.35 | 175 | 20 |
100.00%
|
50.00%
|
| Primer 1535 | Holdemania filiformis | TCGCCTTAAACGGTGCGT | ACCCATGTTCCTGCGCAA | 59.97 | 59.88 | 234 | 20 |
100.00%
|
50.00%
|
| Primer 1536 | Holdemania filiformis | TCGCCTTAAACGGTGCGT | AACCCATGTTCCTGCGCA | 59.97 | 59.88 | 235 | 20 |
100.00%
|
50.00%
|
| Primer 1537 | Holdemania filiformis | TCCTTTGTCGGCAAGCGT | AACGTCAGCGATGCAAGC | 59.89 | 59.14 | 180 | 20 |
100.00%
|
50.00%
|
| Primer 1538 | Holdemania filiformis | CCGGACAAATCGCCTGGAT | ACTGCAGCTGTTGTGCGA | 60.15 | 60.20 | 195 | 17 |
100.00%
|
50.00%
|
| Primer 1539 | Holdemania filiformis | TCGAGCATGTCAACCTGGC | TGACCGTTACGTTGGCGA | 60.38 | 59.27 | 159 | 17 |
100.00%
|
50.00%
|
| Primer 1540 | Holdemania filiformis | TCTTGGCAACCAACCCGA | TGCCGATCATGACGCACT | 59.08 | 59.42 | 192 | 17 |
100.00%
|
50.00%
|